The inhibitory effect of opioid on the hyperpolarization-activated cation currents in rat substantia gelatinosa neurons
The Korean Journal of Physiology and Pharmacology
; : 373-380, 2001.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-727414
Biblioteca responsável:
WPRO
ABSTRACT
The action of opioid on the hyperpolarization-activated cation current (Ih) in substantia gelatinosa neurons were investigated by using whole-cell voltage-clamp recording in rat spinal brain slices. Hyperpolarizing voltage steps revealed slowly activating currents in a subgroup of neurons. The half-maximal activation and the reversal potential of the current were compatible to neuronal Ih. DAMGO (1 muM), a selective-opioid agonist, reduced the amplitude of Ih reversibly. This reduction was dose-dependent and was blocked by CTOP (2 muM), a selective mu-opioid antagonist. DAMGO shifted the voltage dependence of activation to more hyperpolarized potential. Cesium (1 mM) or ZD 7288 (100 muM) blocked Ih and the currents inhibited by cesium, ZD 7288 and DAMGO shared a similar time and voltage dependence. These results suggest that activation of mu-opioid receptor by DAMGO can inhibit Ih in a subgroup of rat substantia gelatinosa neurons.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Substância Gelatinosa
/
Encéfalo
/
Césio
/
Ala(2)-MePhe(4)-Gly(5)-Encefalina
/
Neurônios
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
2001
Tipo de documento:
Artigo