Involvement of NAD (P) H Oxidase in a Potential Link between Diabetes and Vascular Smooth Muscle Cell Proliferation
The Korean Journal of Physiology and Pharmacology
; : 103-110, 2003.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-727611
Biblioteca responsável:
WPRO
ABSTRACT
The cellular mechanisms that contribute to the acceleration of atherosclerosis in diabetes are poorly understood. Therefore, the potential mechanisms involved in the diabetes-dependent increase in vascular smooth muscle cell (VSMC) proliferation was investigated. Using primary culture of VSMC from streptozotocin-induced diabetic rat aorta, cell proliferation assay showed two-fold increase in cell number accompanied with enhanced superoxide generation compared to normal VSMC, 2 days after plating. Both the increased superoxide production and cell proliferation in diabetic VSMC were significantly attenuated by not only tiron (1 mM), a superoxide scavenger, but also by diphenyleneiodonium (DPI; 10micrometer), an NAD (P) H oxidase inhibitor. NAD (P) H oxidase activity in diabetic VSMC was significantly higher than that in control cell, accompanied with increased mRNA expression of p22phox, a membrane subunit of oxidase. Furthermore, inhibition of p22phox expression by transfection of antisense p22phox oligonucleotides into diabetic VSMC resulted in a decrease in superoxide production, which was accompanied by a significant inhibition of cell proliferation. Based on these results, it is suggested that diabetes-associated increase in NAD (P) H oxidase activity via enhanced expression of p22phox contributes to augmented VSMC proliferation in diabetic rats.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligonucleotídeos
/
Aorta
/
Oxirredutases
/
Sal Dissódico do Ácido 1,2-Di-Hidroxibenzeno-3,5 Dissulfônico
/
RNA Mensageiro
/
Transfecção
/
Contagem de Células
/
Superóxidos
/
Proliferação de Células
/
Aterosclerose
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
2003
Tipo de documento:
Artigo