Role of phospholipase A2 in oxidant-induced alteration in phosphate transport in primary cultured rabbit renal proximal tubule cells
The Korean Journal of Physiology and Pharmacology
; : 601-609, 1998.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-727756
Biblioteca responsável:
WPRO
ABSTRACT
The present study was undertaken to examine the role of phospholipase A2 (PLA2) in oxidant-induced inhibition of phosphate transport in primary cultured rabbit renal proximal tubule cells. Uptakes of phosphate and glucose were dose-dependently inhibited by an oxidant t-butylhydroperoxide (tBHP), and the significant inhibition appeared at 0.025 mM of tBHP, whereas tBHP-induced alterations in lipid peroxidation and cell viability were seen at 0.5 mM. tBHP stimulated arachidonic acid (AA) release in a dose-dependent fashion. A PLA2 inhibitor mepacrine prevented tBHP-induced AA release, but it did not alter the inhibition of phosphate uptake and the decrease in cell viability induced by tBHP. tBHP-induced inhibition of phosphate transport was not affected by a PKC inhibitor, staurosporine. tBHP at 0.1 mM did not produce the inhibition of Na+-K+-ATPase activity in microsomal fraction, although it significantly inhibited at 1.0 mM. These results suggest that tBHP can inhibit phosphate uptake through a mechanism independent of PLA2 activation, irreversible cell injury, and lipid peroxidation in primary cultured rabbit renal proximal tubular cells.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfolipases
/
Quinacrina
/
Peroxidação de Lipídeos
/
Sobrevivência Celular
/
Ácido Araquidônico
/
Estaurosporina
/
Terc-Butil Hidroperóxido
/
Fosfolipases A2
/
Glucose
Idioma:
Inglês
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
1998
Tipo de documento:
Artigo