Scopoletin from Cirsium setidens Increases Melanin Synthesis via CREB Phosphorylation in B16F10 Cells
The Korean Journal of Physiology and Pharmacology
; : 307-311, 2014.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-728464
Biblioteca responsável:
WPRO
ABSTRACT
In this study, we isolated scopoletin from Cirsium setidens Nakai (Compositae) and tested its effects on melanogenesis. Scopoletin was not toxic to cells at concentrations less than 50 microM and increased melanin synthesis in a dose-dependent manner. As melanin synthesis increased, scopoletin stimulated the total tyrosinase activity, the rate-limiting enzyme of melanogenesis. In a cell-free system, however, scopoletin did not increase tyrosinase activity, indicating that scopoletin is not a direct activator of tyrosinase. Furthermore, Western blot analysis showed that scopoletin stimulated the production of microphthalmia-associated transcription factor (MITF) and tyrosinase expression via cAMP response element-binding protein (CREB) phosphorylation in a dose-dependent manner. Based on these results, preclinical and clinical studies are needed to assess the use of scopoletin for the treatment of vitiligo.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosforilação
/
Escopoletina
/
Vitiligo
/
Sistema Livre de Células
/
Western Blotting
/
Monofenol Mono-Oxigenase
/
Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico
/
Cirsium
/
Fator de Transcrição Associado à Microftalmia
/
Melaninas
Idioma:
Inglês
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
2014
Tipo de documento:
Artigo