Paracrine influence of human perivascular cells on the proliferation of adenocarcinoma alveolar epithelial cells
The Korean Journal of Physiology and Pharmacology
; : 161-168, 2017.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-728583
Biblioteca responsável:
WPRO
ABSTRACT
Understanding the crosstalk mechanisms between perivascular cells (PVCs) and cancer cells might be beneficial in preventing cancer development and metastasis. In this study, we investigated the paracrine influence of PVCs derived from human umbilical cords on the proliferation of lung adenocarcinoma epithelial cells (A549) and erythroleukemia cells (TF-1α and K562) in vitro using Transwell® co-culture systems. PVCs promoted the proliferation of A549 cells without inducing morphological changes, but had no effect on the proliferation of TF-1α and K562 cells. To identify the factors secreted from PVCs, conditioned media harvested from PVC cultures were analyzed by antibody arrays. We identified a set of cytokines, including persephin (PSPN), a neurotrophic factor, and a key regulator of oral squamous cell carcinoma progression. Supplementation with PSPN significantly increased the proliferation of A549 cells. These results suggested that PVCs produced a differential effect on the proliferation of cancer cells in a cell-type dependent manner. Further, secretome analyses of PVCs and the elucidation of the molecular mechanisms could facilitate the discovery of therapeutic target(s) for lung cancer.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cordão Umbilical
/
Técnicas In Vitro
/
Carcinoma de Células Escamosas
/
Adenocarcinoma
/
Leucemia Eritroblástica Aguda
/
Citocinas
/
Meios de Cultivo Condicionados
/
Técnicas de Cocultura
/
Células K562
/
Células Epiteliais
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
2017
Tipo de documento:
Artigo