Optimal Medium Conditions for the Detection of Cellulolytic Activity in Ganoderma lucidum
Mycobiology
; : 313-316, 2009.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-729425
Biblioteca responsável:
WPRO
ABSTRACT
To determine the optimal medium conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on the chromogenic reaction, we also made and tested various media spanning acidic and alkaline pHs, ranging from 4.5 to 8.0. Furthermore, in order to research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to 35degrees C. On the whole, the best protocol called for Ganoderma lucidum transfer onto media containing Congo red with pH adjusted to 7.0, followed by incubation at 25degrees C for 5 days. Our results will be useful to researchers who aim to study extracellular enzyme activity in Ganoderma lucidum.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenolsulfonaftaleína
/
Azul Tripano
/
Benzenossulfonatos
/
Vermelho Congo
/
Reishi
/
Ganoderma
/
Diminazena
/
Corantes
/
Fungos
/
Concentração de Íons de Hidrogênio
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2009
Tipo de documento:
Artigo