Chinese Cabbage Clubroot Pathogen, Plasmodiophora brassicae, Is Genetically Stable
Mycobiology
; : 225-229, 2009.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-729442
Biblioteca responsável:
WPRO
ABSTRACT
Single spore isolates of Plasmodiophora brassicae e4 and e9 obtained from diseased Chinese cabbage were identified as race 4 and race 9, respectively, by the Williams' differential variety set. To confirm the possibility of variation in same generation and progeny of a single spore isolate of P. brassicae, random amplified polymorphic DNA (RAPD) analysis was conducted using the URP 3, 6 and OPA 7 primers. There was no difference in band type at each part of the gall of Chinese cabbage obtained by inoculation of e4 and e9 and amplification using the URP 3 and 6 primers when the same generation was analyzed. In addition, the progeny analysis, which was expanded to the third generation and conducted using the URP 3 and OPA 7 primers, revealed no differences in the band type of the e4 isolate. Based on these results, the single spore isolate of P. brassicae was genetically stable.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Esporos
/
Variação Genética
/
Brassica
/
DNA
/
Grupos Raciais
/
Povo Asiático
/
Plasmodioforídeos
Limite:
Humanos
Idioma:
Inglês
Revista:
Mycobiology
Ano de publicação:
2009
Tipo de documento:
Artigo