Functional Characterization of cAMP-Regulated Gene, CAR1, in Cryptococcus neoformans
Mycobiology
; : 26-32, 2010.
Article
em En
| WPRIM
| ID: wpr-729552
Biblioteca responsável:
WPRO
ABSTRACT
The cyclic AMP (cAMP) pathway plays a major role in growth, sexual differentiation, and virulence factor synthesis of pathogenic fungi. In Cryptococcus neoformans, perturbation of the cAMP pathway, such as a deletion in the gene encoding adenylyl cyclase (CAC1), causes defects in the production of virulence factors, including capsule and melanin production, as well as mating. Previously, we performed a comparative transcriptome analysis of the Ras- and cAMP- pathway mutants, which revealed 163 potential cAMP-regulated genes (38 genes at a 2-fold cutoff). The present study characterized the role of one of the cAMP pathway-dependent genes (serotype A identification number CNAG_ 06576.2). The expression patterns were confirmed by Northern blot analysis and the gene was designated cAMP-regulated gene 1 (CAR1). Interestingly, deletion of CAR1 did not affect biosynthesis of any virulence factors and the mating process, unlike the cAMP-signaling deficient cac1Delta mutant. Furthermore, the cac1Delta mutant exhibited wild-type levels of the stress-response phenotype against diverse environmental cues, indicating that Car1, albeit regulated by the cAMP-pathway, is not essential to confer a cAMP-dependent phenotype in C. neoformans.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Fenótipo
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Diferenciação Sexual
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Adenilil Ciclases
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Northern Blotting
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AMP Cíclico
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Perfilação da Expressão Gênica
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Cryptococcus
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Cryptococcus neoformans
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Sinais (Psicologia)
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Fatores de Virulência
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Mycobiology
Ano de publicação:
2010
Tipo de documento:
Article