Regulatory T cells for long-lasting effect on asthmatic children caused by dermatophagoides farinae with sublingual immunotherapy / 中华实用儿科临床杂志

ABSTRACT

Objective To investigate the role of regulatory T cells for long-lasting effect of the sublingual immunotherapy(SLIT) in children with allergic asthma caused by dermatophagoides farinae.Methods Sixty children with mild allergic asthma who showed positive skin reaction to dermatophagoides allergen skin test were selected and randomly divided into SLIT group and non-SLIT group.The non-SLIT group received drug therapy only,while the SLIT group received sublingual dermatophagoides farina agent besides drug therapy.The mononuclear cells in the peripheral blood,the regulatory T cells induced by dermatophagoides farinae stimulator in 2 groups were determined by flow cytometry,as well as the levels of the serum IgE in the blood with specific dermatophagoides farina before treatment,1 year after treatment,at the end of treatment,and 1 year after treatment.Results The levels of regulatory T cells in the SLIT group were increased,with the treatment of specific immunotherapy it increased further and reached the peak after oneyear treatment and the peak level maintained after one year of treatment,which was not significantly different in the levels of regulatory T cells at the same period of time in the non-SLIT group (P ＞ 0.05).Compared with the non-SLIT group,the level of serum IgE had no significant differences between 2 groups before and after treatment (P ＞ 0.05).Conclusion SLIT may induce immune tolerance by up-regulating inductive regulatory T cells,which may be involved in therapeutic mechanisms of the SLIT in the patients with allergic asthma caused by dust mites.