Diagnostic value of plasma heparin-binding protein and pentraxin 3 in neonatal bacterial infection diseases / 中华检验医学杂志

ABSTRACT

Objective To evaluate the diagnosis value of heparin-binding protein ( HBP ) and pentraxin 3 ( PTX3 ) in neonatal bacterial infectious diseases . Methods A retrospective study was conducted on 30 septic neonatal as neonatal sepsis group and 84 local infection neonatal as general infection group from May to November 2017 in Renmin Hospital of Wuhan University .It also selected 50 high bilirubin hematic disease but without infection or shock neonatal ( control group ) .A total of 114 neonatal bacterial infection ( neonatal sepsis group and general infection group ) were divided into shock group ( 39 cases) and non-shock group ( 75 cases ) . The levels of plasma HBP and PTX3 were tested with immunoturbidimetry and ELSIA respectively .The results of procalcitonin ( PCT ) and white blood cells (WBC) counts were collected.Non-parametric test were performed for non-normal distribution data; the diagnostic performances of data were evaluated by receiver operating characteristic ( ROC) curve; pearson correlation coefficient was performed for correlation analysis .Results Plasma levels of HBP in neonatal sepsis group, general infection group and control group were (64.41 ±78.51) ng/ml, (47.16 ±50.59) ng/ml and (31.97 ±20.76) ng/ml, respectively; plasma levels of PTX3 were (2.23 ±1.44) ng/ml, (1.76 ±0.94) ng/ml and (1.26 ±0.66) ng/ml, respectively;serum levels of PCT were (31.92 ±36.65) ng/ml,( 7.72 ±9.28 ) ng/ml and ( 1.87 ±5.02 ) ng/ml, respectively.The levels of PTX3 and PCT in neonatal sepsis group were significantly higher than in general infection group (Z=3.74, Z=5.01, all P<0.05) and control group (Z=3.98, Z=5.20, all P<0.05).The levels of HBP in neonatal sepsis group were significantly higher than in control group ( Z =2.37, P <0.05 ), but there were no significant difference in neonatal sepsis group and general infection group (Z=1.16, P>0.05).The levels of PTX3 and PCT in shock group were significantly higher than in non-shock group ( Z=2.20, Z=3.70, all P<0.05), but there were no significant difference in plasma HBP of shock and non-shock group ( Z=0.37, P>0.05).The area under curve (AUC) of HBP, PTX3 and PCT were 0.683, 0.802 and 0.869 respectively in the diagnosis of neonatal bacterial infection diseases .The biggest AUC of combined diagnosis of HBP, PTX3 and PCT was 0.910.There was a positive correlation between PTX 3 and PCT ( r=0.242, P<0.05) .Conclusions PTX3 and PCT could probably be acted as an important biomarker for diagnosis of neonatal bacterial infection diseases , and combined diagnosis of HBP , PTX3 and PCT could be superior to single biomarker diagnosis.