Gene Expression Profile of Olfactory Transduction Signaling in an Animal Model of Human Multiple Sclerosis
Experimental Neurobiology
; : 74-84, 2019.
Article
em En
| WPRIM
| ID: wpr-739529
Biblioteca responsável:
WPRO
ABSTRACT
Olfactory dysfunction occurs in multiple sclerosis in humans, as well as in an animal model of experimental autoimmune encephalomyelitis (EAE). The aim of this study was to analyze differentially expressed genes (DEGs) in olfactory bulb of EAE-affected mice by next generation sequencing, with a particular focus on changes in olfaction-related signals. EAE was induced in C57BL/6 mice following immunization with myelin oligodendrocyte glycoprotein and adjuvant. Inflammatory lesions were identified in the olfactory bulbs as well as in the spinal cord of immunized mice. Analysis of DEGs in the olfactory bulb of EAE-affected mice revealed that 44 genes were upregulated (and which were primarily related to inflammatory mediators), while 519 genes were downregulated; among the latter, olfactory marker protein and stomatin-like 3, which have been linked to olfactory signal transduction, were significantly downregulated (log2 [fold change] >1 and p-value < 0.05). These findings suggest that inflammation in the olfactory bulb of EAE-affected mice is associated with the downregulation of some olfactory signal transduction genes, particularly olfactory marker protein and stomatin-like 3, which may lead to olfactory dysfunction in an animal model of human multiple sclerosis.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Bulbo Olfatório
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Medula Espinal
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Transdução de Sinais
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Regulação para Baixo
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Expressão Gênica
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Imunização
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Modelos Animais
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Encefalomielite Autoimune Experimental
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Proteína de Marcador Olfatório
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Transcriptoma
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
Experimental Neurobiology
Ano de publicação:
2019
Tipo de documento:
Article