Differentiation potential of canine mesenchymal stem cells on hydrogel scaffold-based three-dimensional environment / 대한수의학회지
Korean Journal of Veterinary Research
; : 211-217, 2018.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-741518
Biblioteca responsável:
WPRO
ABSTRACT
Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy. Physiological cell environment not only connects cells to each other, but also connects cells to the extracellular matrix that provide mechanical support, thus exposing the entire cell surface and activating signaling pathways. Hydrogel is a polymeric material that swells in water and maintains a distinct 3-dimensional (3D) network structure by cross linking. In this study, we investigated the optimized cellular function for canine adipose tissue-derived MSCs (cAD-MSCs) using hydrogel. We observed that the expression levels of Ki67 and proliferating cell nuclear antigen, which are involved in cell proliferation and stemness, were increased in transwell-hydrogel (3D-TN) compared to the transwell-normal (TN). Also, transforming growth factor-β1 and SOX9, which are typical bone morphogenesis-inducing factors, were increased in 3D-TN compared to the TN. Collagen type II alpha 1, which is a chondrocyte-specific marker, was increased in 3D-TN compared to the TN. Osteocalcin, which is a osteocyte-specific marker, was increased in 3DTN compared to the TN. Collectively, preconditioning cAD-MSCs via 3D culture systems can enhance inherent secretory properties that may improve the potency and efficacy of MSCs-based therapies for bone regeneration process.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteogênese
/
Polímeros
/
Regeneração Óssea
/
Água
/
Osteocalcina
/
Antígeno Nuclear de Célula em Proliferação
/
Hidrogéis
/
Condrogênese
/
Colágeno Tipo II
/
Engenharia Tecidual
Idioma:
Coreano
Revista:
Korean Journal of Veterinary Research
Ano de publicação:
2018
Tipo de documento:
Artigo