A simple method of separating outer hair cells in Guinea pig / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 829-831, 2008.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-746541
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#In order to meet the needs of electrophysiology outer hair cells, we investigate a method to separate outer hair cells simply and effectively.@*METHOD@#The basal membrane was dissected combined with spiral ligament and axis of cochlea, then incubated with enzyme, and then mechanically triturated by micropipette.@*RESULT@#A large number of living outer hair cells were obtained. Of 80% cells could keep good condition in 6 hours.@*CONCLUSION@#If increasing the enzyme concentration slightly and the large part of cochlea without outer bone was enzymes, we can get living outer hair cells easily.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Separação Celular
/
Cóclea
/
Biologia Celular
/
Cobaias
/
Células Ciliadas Auditivas
/
Métodos
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2008
Tipo de documento:
Artigo