Long Non-Coding RNA LINC00525 Promotes the Stemness and Chemoresistance of Colorectal Cancer by Targeting miR-507/ELK3 Axis
International Journal of Stem Cells
; : 347-359, 2019.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-764069
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND AND OBJECTIVES:
This study aims to explore the effects of a long non-coding RNA, LINC00525, on colorectal cancer (CRC) and its underlying molecular mechanisms.METHODS:
The qPCR, MTT, colony formation, Western blotting, Luciferase reporter and biotin pull-down, shRNA knockdown and DNA fragmentation assays were performed in this study.RESULTS:
High expressions of LINC00525 were associated with poor prognosis of CRC patients. LINC00525 knockdown decreased stemness properties and increased sensitivities to oxaliplatin. MiR-507 was a direct target of LINC00525 and overexpression of miR-507 significantly decreased abilities of tumorsphere formation and cell growth. Overexpression of miR-507 resulted in a decrease of expression of cancer stem cell markers and the increase of apoptosis rates. MiR-507 regulated the expression of ELK3. In addition, LINC00525 knockdown decreased the expression of ELK3. Restoration of ELK3 expression abrogated the effects of LINC00525 knockdown. LINC00525 could be served as prognostic marker of CRC.CONCLUSIONS:
LINC00525 enhanced stemness properties and increased sensitivities of CRC cells to oxaliplatin by targeting miR-507/ELK3 axis.
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Neoplasias Colorretais
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Prognóstico
/
Células-Tronco Neoplásicas
/
Biotina
/
Neoplasias Colorretais
/
Western Blotting
/
Apoptose
/
RNA Interferente Pequeno
/
Fragmentação do DNA
/
RNA Longo não Codificante
/
Luciferases
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
International Journal of Stem Cells
Ano de publicação:
2019
Tipo de documento:
Artigo