Mitigation effects of red Platycodon grandiflorum extract on lipopolysaccharide-induced inflammation in splenocytes isolated from mice / 한국영양학회지
Journal of Nutrition and Health
; : 243-249, 2019.
Article
em Ko
| WPRIM
| ID: wpr-765986
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: Platycodon grandiflorum (PG) is known to have effective antimicrobial and anticancer activity. The main bioactive components of PG are saponins, and these could contribute to anti-inflammatory activity. However, little is known about the anti-inflammatory effect of PG. In this study, we aim to assess the anti-inflammatory response to Red PG Extract (RPGE) in splenocytes under ex vivo conditions. METHODS: The cell viability of isolated splenocytes taken from mice was analyzed by performing a Cell Counting Kit-8 assay. The productions of nitric oxide (NO) and cytokines (specifically interleukin-6 (IL-6) and interleukin-10 (IL-10)) were measured utilizing Griess reagent and ELISA, respectively. RESULTS: We found that co-treatment with RPGE and Lipopolysaccharide (LPS) decreased isolated splenocyte proliferation as compared with that of the LPS-stimulated control. We also observed that RPGE markedly suppressed NO synthesis and IL-6 production that was induced by LPS. There were no significant differences of IL-10 production between co-treatment with RPGE plus LPS and treatment with LPS alone. CONCLUSION: When taken together, our data has shown that RPGE mitigates LPS-induced inflammation in splenocytes isolated from mice. Further research is surely needed to confirm the anti-inflammation effects of RPGE in an in vivo model.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Saponinas
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Ensaio de Imunoadsorção Enzimática
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Contagem de Células
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Sobrevivência Celular
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Citocinas
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Interleucina-6
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Interleucina-10
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Platycodon
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Inflamação
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Óxido Nítrico
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Ko
Revista:
Journal of Nutrition and Health
Ano de publicação:
2019
Tipo de documento:
Article