Construction of yeast two-hybrid library of Salvia miltiorrhiza and screening of SmJAZ8 interaction protein / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 484-492, 2018.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-771711
Biblioteca responsável:
WPRO
ABSTRACT
The study is aimed to construct high quality Salvia miltiorrhiza cDNA library and obtain the SmJAZ8 gene of S. miltiorrhiza by yeast two-hybrid system. In this study, full-length cDNA was synthesized from roots, stems, leaves, flowers and hairy roots of S. miltiorrhiza. The full-length cDNA library was synthesized by SMART method and constructed with DSN homogenization technique. The results showed that the library capacity was 1.45×10⁶, the recombination rate was 100%, and the average size of the insert was 500-2 000 bp. The recombinant vector of pDEST-pGADT7-SmJAZ8 was constructed and transformed into Y2HGold strain. The interaction protein was screened by yeast two-hybrid system. The DnaJ protein and UBQ protein were screened by yeast two-hybrid system. This study has successfully constructed a full-length cDNA library of S. miltiorrhiza, and laid the foundation for the follow-up study on functional gene screening and gene function of S. miltiorrhiza.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas de Plantas
/
Biblioteca Gênica
/
DNA Complementar
/
Técnicas do Sistema de Duplo-Híbrido
/
Salvia miltiorrhiza
/
Proteínas Correpressoras
/
Genética
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
/
Estudo prognóstico
/
Estudo de rastreamento
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2018
Tipo de documento:
Artigo