Detection of mutant dystrophin gene carrier using quantitative Polymerase Chain Reaction
Journal of Medical Research
; : 1-10, 2008.
Artigo
em Vietnamês
| WPRIM (Pacífico Ocidental)
| ID: wpr-775
Biblioteca responsável:
WPRO
ABSTRACT
Background:
Deletion and duplication mutations of dystrophin gene make up from 70 to 75% of patients with Duchenne Muscular Dystrophy (DMD). Two thirds of children with DMD inherited from the heterozygous mothers the mutated gene which is located on one of the sex chromosomes.Objective:
To detect the asymptomatic carriers of dystrophin gene mutation using molecular techniques. Subject andmethods:
3 DMD patients and their 9 relatives. Using techniques DNA extraction and quantitative Polymerase Chain Reaction (PCR).Results:
Successfully detected 4 heterozygous individuals from 9 female members of three different families that have already confirmed DMD patients.Conclusion:
This method could lead to a new way of prenatal diagnosis of DMD as well as other genetic disorders that are caused by deletion or duplication mutation.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Distrofia Muscular de Duchenne
Tipo de estudo:
Estudo diagnóstico
Idioma:
Vietnamês
Revista:
Journal of Medical Research
Ano de publicação:
2008
Tipo de documento:
Artigo