The protective effects of vitamin E on lung injury caused by high temperature and PM in COPD rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 293-296, 2019.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-776512
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effects of vitamin E on the respiratory function impairment in rats with chronic obstructive pulmonary disease (COPD) after exposed to high temperature and PM.@*METHODS@#Fifty-four 7-week-old SPF male Wistar rats were randomly divided into 9 experimental groups (n=6). The rat COPD model was established by lipopolysaccharide (LPS) and smoke exposure. After modeled, the rats were tracheal instilled with PM (0 mg/ml, 3.2 mg/ml) and intraperitoneally injected with vitamin E at the dose of 40 mg/kg (20 mg/ml). Part of rats (high temperature groups) were then exposed to high temperature (40℃), once (8 h) a day for three consecutive days. After the last exposure, the lung function of rats was detected. The expression levels of inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α) and monocyte chemotactic protein-1 (MCP-1) were detected by corresponding ELISA kits.@*RESULTS@#Compared with the control group, exposure of high temperature and PM could inhibit the lung function of COPD rats significantly (P<0.05); the level of MCP-1 was increased significantly in PM-exposure groups (P<0.05); iNOS was increased significantly in the groups of high temperature (P<0.05). Compared with the single-PM exposure groups, TNF-α in lung was decreased in the normal temperature health group and high temperature COPD group (P<0.05) after treated with vitamin E; MCP-1 was decreased in all vitamin E-treated groups (P<0.05); the decreased iNOS only appeared in the group of high temperature with vitamin E treatment.@*CONCLUSION@#High temperature and PM could aggravate the inflammation of COPD rats. As an antioxidant, vitamin E may protect the lung from the damage effects.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
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Vitamina E
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Distribuição Aleatória
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Fator de Necrose Tumoral alfa
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Ratos Wistar
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Quimiocina CCL2
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Doença Pulmonar Obstrutiva Crônica
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Tratamento Farmacológico
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Óxido Nítrico Sintase Tipo II
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Material Particulado
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2019
Tipo de documento:
Artigo