Identification of 23 unknown Li minority medicinal plants based on DNA barcoding / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 283-292, 2019.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-777440
Biblioteca responsável:
WPRO
ABSTRACT
DNA barcode molecular biological technique is used to identify the species of 23 unknown Li minority medicinal plants.DNA was extracted from 23 unknown medicines using the Plant Genomic DNA Extraction kit. The ITS2 and psbA-trnH regions were amplified and sequenced bi-directionally. The Codon Code Aligner V 7. 0. 1 was used to proofread and assemble the contigs and generated consensus sequences. All the sequences were submitted to Traditional Chinese Medicine DNA Barcode Database and NCBI Gen Bank to get information of the species identifications. If the maximum similarity of the identification result is ≥ 97%,exact species can be known. If it is between 97% and 90%,samples' genus can be confirmed; If it is <90%,then we can only confirm its family. Finally there are 17 samples can be identified to species level,5 can be identified to genus level and 1 can be identified to family level. This shows that DNA barcoding used in medicinal plants molecular identification,can identify unknown species rapidly and accurately.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Classificação
/
Análise de Sequência de DNA
/
DNA de Plantas
/
Código de Barras de DNA Taxonômico
/
Genética
/
Medicina Tradicional Chinesa
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2019
Tipo de documento:
Artigo