Analysis of association between different HCV genotypes and serum interleukin-17, interleukin-6, and vitamin D in patients with HCV-related cirrhosis / 临床肝胆病杂志

ABSTRACT

ObjectiveTo investigate the association between different hepatitis C virus (HCV) genotypes and serum interleukin-17 (IL-17), interleukin-6 (IL-6), and vitamin D in patients with HCV-related cirrhosis. MethodsSeventy-six patients with HCV-related cirrhosis, who were admitted to 302 Hospital of PLA from January to December, 2012, were enrolled in the study, and they were divided into type 1b group (n=47) and type 2a group (n=29) according to the genotypes. The levels of serum IL-17, IL-6, and vitamin D were determined by enzyme-linked immunosorbent assay. Comparison between the two groups was made by t test, and Spearman correlation analysis was used to investigate the association between serum IL-17, IL-6, and vitamin D and genotypes. ResultsThe findings in improved Child-Pugh classification showed that there were significant differences in the percentage of grade A patients between type 1b group and type 2a group (χ2=4.97, P＜0.05); when compared with type 2a group, type 1b group showed significantly higher lentiviral titers of alpha-fetoprotein (AFP) and HCV RNA and levels of IL-17, IL-6, and tumor necrosis factor-α (TNFα) (t=21.56, 16.51, 1231, 10.71, and 7.23, respectively, all P＜0.05), but significantly lower levels of interferon-γ (IFNγ), 25(OH)D, and 1,25(OH)2D (t=3.98, 6.32, and 4.88, respectively; all P＜0.05); Spearman correlation analysis showed that the lentiviral titers of AFP and HCV RNA and the levels of IL-17 and IL-6 were positively correlated with genotype 1b and genotype 2a (all P＜0.05), while the levels of IFNγ, TNFα, and 25-OH-D were negatively correlated with genotype 1b and genotype 2a (all P＜0.05). ConclusionCompared with those with genotype 2a, HCV-related cirrhosis patients with genotype 1b have higher serum levels of IL-17, IL-6, AFP, and HCV RNA, but a lower level of vitamin D; the results suggested that there are correlations between serum IL-17, IL-6, 25-OH-D, AFP, and HCV RNA and genotype, but no significant correlation between 1,25(OH)2D and genotype.