Determination of the content of baicalin in Qiguanyan granules by HPLC / 药学实践杂志

ABSTRACT

Objective To establish a method of HPLC for the determination of baicalin in Qiguanyan granules .Methods HPLC was performed on ZORBAX SB-C18 (4 .6 mm × 150 mm ,5 μm) column with mobile phase consisted of methanol-0.1%phosphoric acid (47∶ 53) at ambient temperature .The flow rate was 1 .0 ml/min .The detection wavelength was 280 nm . Results Baicalin showed a good linearity in the range of 6 .63-210 μg/ml(r=0.999 8 ,n= 6) ,and the average recovery was 98.16% (RSD=1.89% ) .Conclusion This methods was simple ,accurate ,sensitive ,reproducible and reliable ,which was suit-able for the content control of Qiguanyan granules .