Study on the three-dimensional chitosan-decellularised-derma scaffolds for bone tissue engineering / 医用生物力学

ABSTRACT

Objective: To observe the adhesion of MC3T3-El osteoblastic progenitor cells to the three-dimensional chitosan-decellularised-derma scaffolds, and evaluate the cytocompatibility of the scaffolds. Methods: The three-dimensional chitosan-decellularised-derma scaffolds were prepared by the freeze-drying method, the porosity, density and water absorption of which were measured. The microscopic morphology of the composite scaffolds was analyzed by the scanning electron microscopy (SEM). The MC3T3-E1 cells cultivated in vitro were seeded onto the composite scaffolds, and then co-cultured for 2, 3, 4 and 5 hours. At each time point, three specimens from each matrix were taken to determine the cell-adhesion rate, in order to ascertain the best time of the cell-adhesion. The cells were seeded onto the composite scaffolds, and then co-cultured for 1, 3, 5, 7, 9, 11 and 13 days. The MC3T3-E1 cells inside were evaluated with MTS test. The cell morphology was observed by the histological staining. The compression tests were performed using a Universal Testing Machine, at room temperature, as compared with no-cell-scaffolds.Results: The three-dimensional chitosan-decellularised-derma scaffolds had high interval porosity with the porosity (92.8%), the density (0.09796g/ml) and the water absorption (2169±100)%. The cytocompatibility test showed that the seeded MC3T3-E1 cells could adhere to the scaffolds and proliferate.Conclusion：The three-dimensional chitosan-decellularised-derma scaffolds had high interval porosity with the well-distributed diameter. The MC3T3-E1 cells were easy to adhere the scaffolds and proliferate which showed that the scaffolds had a good cytocompatibility.