A Study on the establishment of immune thrombocytopenia model induced by anti-platelet GPⅠbα antibodies / 中华血液学杂志
Chinese Journal of Hematology
; (12): 390-393, 2017.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-808745
Biblioteca responsável:
WPRO
ABSTRACT
Objective@#To establish primary immune thrombocytopenia (ITP) animal model induced by anti-platelet membrane glycoprotein GPⅠbα antibodies AN51 and R300.@*Methods@#Twenty guinea pigs (6-8 week) were divided into 4 groups. Five guinea pigs in each group were intravenously injected with different doses of AN51 (0.05, 0.1, 0.2 μg/g) and 0.2 μg/g IgG as control. The whole blood was collected from inner angular venous plexus. Platelets number was determined by an automated cell counter and Swiss-Jim method. Then, the similar protocol was used to establish ITP nude mice model by intraperitoneal injection of different concentrations of anti-platelet GPⅠbα antibody R300, respectively.@*Results@#①Five minutes after intravenous injection of AN51 at 0.05, 0.1 and 0.2 μg/g, the platelet counts of guinea pigs reduced about 0-5%, 50%-60% and 70%-80% compared to the control group, respectively. The difference was statistically significant (P<0.01) . ②Six hours after intraperitoneal injection of R300 at 0.05, 0.1, 0.2 μg/g, the platelet counts of nude mice decreased about 20%-30%, 60%-70% and 80%-90% compared to the control group, respectively. The difference was statistically significant (P<0.01) . The nude mice, injected 0.2 μg/g R300 once a day for 2 weeks, showed typical ITP clinical manifestations including large number of petechiaes or ecchymoses on limbs, head and abdomen.@*Conclusion@#AN51 at 0.2 μg/g and R300 at 0.2 μg/g could establish stable ITP model in guinea pigs and nude mice respectively.
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Base de dados:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Guia de prática clínica
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2017
Tipo de documento:
Artigo