The expression of lncRNA UCA1 and its role on the proliferation and invasion in hepatic cancer and HepG2 cells / 天津医药

ABSTRACT

@# Objective Toinvestigatetheeffectsoflongnon-codingRNAurothelialcarcinoma-associated1(lncRNA UCA1) silencing on cell proliferation and invasion of human hepatic cancer HepG2 cells, and its mechanism thereof. Methods TheexpressionoflncRNAUCA1wasanalyzedbyreal-timePCRin20tumortissueand20adjacentnon-tumor tissuesamplesofhepaticcancer.HepG2cellswasculturedin vitro,andlncRNAUCA1specificshorthairpinRNA(shRNA1 andshRNA2)wastransfected.CCK-8assay,TranswellassayandWound-healingassaywereusedtodetecttheeffectof lncRNAUCA1silencingoncellproliferation,invasionandmigrationofHepG2cells.TheeffectoflncRNAUCA1silencing onproteinandmRNAexpressionofCyclinD1,vascularendothelialgrowthfactor(VEGF),matrixmetalloproteinase(MMP)9,focaladhesionkinase(FAK)andIntegrinβ3onlncRNAUCA1silencingwasmeasuredbyreal-timePCRandWestern blotassay.Results TheexpressionofLncRNAUCA1washigherinhepaticcancertissues.ThesilencingoflncRNAUCA1 significantly inhibited the growth of HepG2 cells, and the abilities of cell invasion and migration were downregulated. Westernblotassayandreal-timePCRshowedthatexpressionsofCyclinD1,VEGF,MMP-9,FAKandIntegrinβ3were significantlyinhibited.Conclusion TheresultssuggestthattheabnormalexpressionoflncRNAUCA1isassociatedwith thedevelopmentofhumanhepaticcancer.ThesilencingoflncRNAUCA1couldsuppressthecellproliferation,invasionand migrationofhepaticcarcinomacells.