Development of a quantitative ELISA kit for human secreted CD306/LAIR-2 and its application / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 847-852, 2014.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-812192
Biblioteca responsável:
WPRO
ABSTRACT
AIM@#A quantitative ELISA kit for the detection of human secreted CD306/LAIR-2 was developed using monoclonal and polyclonal antibodies which were raised against a highly purified recombinant human secreted CD306/LAIR-2.@*METHODS@#Anti-human secreted CD306/LAIR-2 monoclonal and polyclonal antibodies were raised by immunizing mouse or rabbit with recombinant human secreted CD306/LAIR-2. The monoclonal antibody was purified by protein G affinity, whereas the polyclonal antibody was purified by protein A affinity. The best match pair of antibodies were found and used to develop a double antibody sandwich ELISA kit for the detection of human secreted CD306/LAIR-2 in human samples.@*RESULTS@#A human secreted CD306/LAIR-2 ELISA kit was formulated with highly purified recombinant human secreted CD306/LAIR-2, highly specific monoclonal and polyclonal antibodies. This kit realized the quantitative measurement of recombinant human CD306/LAIR-2 and natural CD306/LAIR-2 in human serum samples.@*CONCLUSIONS@#The developed human secreted CD306/LAIR-2 ELISA kit is a reliable quantitation immunoassay kit.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
Proteínas Recombinantes
/
Ensaio de Imunoadsorção Enzimática
/
Receptores Imunológicos
/
Alergia e Imunologia
/
Métodos
/
Camundongos Endogâmicos BALB C
/
Anticorpos Monoclonais
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2014
Tipo de documento:
Artigo