Crebanine inhibits voltage-dependent Na+ current in guinea-pig ventricular myocytes / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 20-23, 2014.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-812314
Biblioteca responsável:
WPRO
ABSTRACT
AIM@#To study the effects of crebanine on voltage-gated Na(+) channels in cardiac tissues.@*METHODS@#Single ventricular myocytes were enzymatically dissociated from adult guinea-pig heart. Voltage-dependent Na(+) current was recorded using the whole cell voltage-clamp technique.@*RESULTS@#Crebanine reversibly inhibited Na(+) current with an IC50 value of 0.283 mmol·L(-1) (95% confidence range 0.248-0.318 mmol·L(-1)). Crebanine at 0.262 mmol·L(-1) caused a negative shift (about 12 mV) in the voltage-dependence of steady-state inactivation of Na(+) current, and retarded its recovery from inactivation, but did not affect its activation curve.@*CONCLUSION@#In addition to blocking other voltage-gated ion channels, crebanine blocked Na(+) channels in guinea-pig ventricular myocytes. Crebanine acted as an inactivation stabilizer of Na(+) channels in cardiac tissues.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Aporfinas
/
Farmacologia
/
Medicamentos de Ervas Chinesas
/
Regulação para Baixo
/
Células Cultivadas
/
Química
/
Biologia Celular
/
Miócitos Cardíacos
/
Stephania
/
Canais de Sódio Disparados por Voltagem
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2014
Tipo de documento:
Artigo