Anti-asthmatic effects of oxymatrine in a mouse model of allergic asthma through regulating CD40 signaling / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 368-374, 2015.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-812533
Biblioteca responsável:
WPRO
ABSTRACT
The aim of the study was to investigate the anti-asthmatic effects of oxymatrine (OXY) and the possible underlying mechanisms. The mouse asthma model was established by ovalbumin (OVA) intraperitoneal injection. A total of fifty mice were randomly assigned to five groups control, OVA, OVA + dexamethasone (Dex, 2 mg · kg(-1)), and OVA + OXY (40 mg · kg(-1)), and OVA + OXY (80 mg · kg(-1)), respectively. Histological studies were conducted by the hematoxylin and eosin (HE) staining, the levels of interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-13, and IgE were evaluated by enzyme-linked immunosorbent assay (ELISA), and the protein level of CD40 was analyzed by Western blotting. OXY inhibited OVA-induced increases in eosinophil count; the levels of IL-4, IL-5, IgE, and IL-13 were recovered. It also substantially inhibited OVA-induced eosinophilia in lung tissues and the expression of CD40 protein. These findings suggest that OXY may effectively ameliorate the progression of asthma and could be explored as a possible therapy for patients with allergic asthma.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Eosinofilia Pulmonar
/
Quinolizinas
/
Asma
/
Imunoglobulina E
/
Dexametasona
/
Ensaio de Imunoadsorção Enzimática
/
Líquido da Lavagem Broncoalveolar
/
Transdução de Sinais
/
Distribuição Aleatória
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2015
Tipo de documento:
Artigo