Identification, structure and function of a novel tetraspaninhomologue from Spirometra erinaceieuropaei
Asian Pacific Journal of Tropical Medicine
; (12): 739-742, 2011.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-820065
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To identify a full length c DNA sequence of a novel tetraspanin (TSP) homologue from Spirometra erinaceieuropaei and to predict the structure and function of its encoding protein using bioinformatics methods.@*METHODS@#Using the NCBI, EMBI, Expasy and other online sites, the open reading frame (ORF), conserved domain, physical and chemical parameters, signal peptide, transmembrane domain, epitope, topological structures of the protein sequences were predicted. And Vector NTI software was used for multiple sequence alignment and phylogenetic tree construction.@*RESULTS@#The target sequence was 1132 bp length with a 681 bpbiggest ORF encoding 226 amino acids protein with typical TSP conserved domain. It was confirmed as full length c DNA of TSP16 from Spirometra erinaceieuropaei and named as SeTSP16 (GenBank accession number JF728872). The predicted molecular weight and isoelectric point of the deduced protein were 24 750.5 Da and 7.88 Da, respectively. Compared with TSP16s from Schistosoma japonicum and Schistosoma mansoni, it showed similarity of 59% and 59%, respectively. SeTSP16 contained four transmembrane domains (TM1-4), intracellular N and C-termini, one short small extracellular loop and one large extracellular loop. Four major epitopes that were significant different from the corresponding epitope regions of TSP16 from Schistosoma mansoni and Schistosoma japonicum were predicted.@*CONCLUSIONS@#The full length c DNA sequences of SeTSP16 are identified. It encodes a transmembrane protein which might be an ideal diagnosis antigen and target molecule for antiparasitic drugs.
Texto completo:
Disponível
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Zoonoses
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Spirometra
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Proteínas de Helminto
/
Química
/
Alinhamento de Sequência
/
Sequência de Aminoácidos
/
Classificação
/
Clonagem Molecular
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2011
Tipo de documento:
Artigo