Free radical scavenging property and antiproliferative activity of Rhodiola imbricata Edgew extracts in HT-29 human colon cancer cells
Asian Pacific Journal of Tropical Medicine
; (12): 11-19, 2013.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-820574
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the in vitro antioxidant and antiproliferative activity of rhizome extracts of Rhodiola imbricata (R. imbricata) in HT-29 human colon cancer cell line.@*METHODS@#The successively extracted rhizome of R. imbricata using various solvents was analyzed for their total phenolics, tannins and flavonoid contents. In vitro antioxidant activity was evaluated by employing different assays, including DPPH, ABTS radical scavenging assays, FRAP, phosphomolybdenum reduction assay, superoxide anion, hydroxyl radical scavenging activities and metal chelating ability.@*RESULTS@#Acetone and methanol extracts recorded higher phenolic content and showed comparable antioxidant activity with standard reference. Additionally, they also inhibited the proliferation of HT-29 cells upon treatment at higher concentration (200 μg/mL) (acetone and methanol, 84% and 84%, respectively). On examination acetone extract exhibited antiproliferative activity in a concentration dependent manner whereas, methanol extract showed both dose dependent and time dependent inhibitory activity.@*CONCLUSIONS@#The results obtained justify the traditional usage of R. imbricata from their promising antioxidant activity.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Extratos Vegetais
/
Química
/
Análise de Variância
/
Sequestradores de Radicais Livres
/
Neoplasias do Colo
/
Folhas de Planta
/
Células HT29
/
Rhodiola
Limite:
Humanos
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2013
Tipo de documento:
Artigo