Production of L-2-aminobutyric acid from L-threonine using a trienzyme cascade / 生物工程学报
Chinese Journal of Biotechnology
; (12): 782-791, 2020.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-826898
Biblioteca responsável:
WPRO
ABSTRACT
L-2-aminobutyric acid (L-ABA) is an important chemical raw material and chiral pharmaceutical intermediate. The aim of this study was to develop an efficient method for L-ABA production from L-threonine using a trienzyme cascade route with Threonine deaminase (TD) from Escherichia. coli, Leucine dehydrogenase (LDH) from Bacillus thuringiensis and Formate dehydrogenase (FDH) from Candida boidinii. In order to simplify the production process, the activity ratio of TD, LDH and FDH was 110.2 after combining different activity ratios in the system in vitro. The above ratio was achieved in the recombinant strain E. coli 3FT+L. Moreover, the transformation conditions were optimized. Finally, we achieved L-ABA production of 68.5 g/L with a conversion rate of 99.0% for 12 h in a 30-L bioreactor by whole-cell catalyst. The environmentally safe and efficient process route represents a promising strategy for large-scale L-ABA production in the future.
Texto completo:
Disponível
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Treonina
/
Treonina Desidratase
/
Bacillus thuringiensis
/
Candida
/
Escherichia coli
/
Leucina Desidrogenase
/
Formiato Desidrogenases
/
Aminobutiratos
/
Metabolismo
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2020
Tipo de documento:
Artigo