Effects of U75302, blocker of leukotriene B4 receptor 1, on cellular immunity and inflammation in septic mice / 第二军医大学学报

ABSTRACT

Objective To observe the influence of U75302, blocker of leukotriene B4 receptor 1, on immune function in septic mice, so as to explore the implication of blocking leukotriene B4 receptor 1 for treatment of sepsis. Methods Experimental sepsis model was induced by cecal ligation and puncture (CLP). A total of 18 male C57BL/6 mice were randomly divided into sham group (n=6), CLP group (n=6) and CLP + U75302 intraperitoneal injection group (n = 6). The peripheral blood cytokine tumor necrosis factor- α (TNF-α) and interleukin-10 (IL-10) levels, peritoneal lavage fluid Gr-1+ cell count and thymus T lymphocytes apoptosiswere detected in mice of three groups 24 h after surgery. Results Compared with the CLP group, CLP + U75302 intraperitoneal injection group had significantly reduced blood TNF-α level (by 43%, P< 0. 05), significantly increased IL-10 levll (by 88%, P<0. 05), signficantly decreased Gr-1+ cell counts in peritoneal lavage fluid (P<0. 05), and significantly decreased apoptosis of thymus T lymphocytes (P<0. 01). Conclusion Blocking leukotriene B4 receptor 1 with U75302 may decrease peripheral TNF-α level, increase IL-10 level, and improve cellular immunity, which may be involved in inflammation of sepsis.