Protective effect of hydrogen-lactated Ringer's solution against extensive burn-induced intestine injury in rats after delayed fluid resuscitation / 第二军医大学学报

ABSTRACT

Objective: To investigate the protective effect of hydrogen-rich lactated Ringer's solution (HRS) against intestine injury induced by extensive burn in Sprague-Dewley (SD) rat model after delayed fluid resuscitation. Methods: Thirty-six male SD rats were randomly divided into three groups (n=12): sham-burn group(S group), burn plus normal lactated Ringer's solution(NRS) group (BR group) and burn plus hydrogen-rich lactated Ringer's solution(HRS) group (BH group). Rats in the S group were immersed into 37°C water without fluid replacement; those in BR group and BH group were subjected to 30% total body surface area (TBSA) m degree full-thickness scald. At first, a total of 2 ml · (1% TBSA)-1 · kg-1 of HRS or NRS were replaced at7h post-burn and half volume of the total solution was replaced at the 9 h and 17 h. All rats were sacrificed at 24 h. Small intestine tissues were removed forH-E staining and determination of MDA content, MPO and SOD activity; and the levels of the IL-1ß and TNF-α were determined by ELISA. Results: The intestinal injury was relieved in BH group compared with BR group. The intestinal MDA content (P<0. 05) and activities of MPO and SOD (P<0. 01) were all decreased in BH group. IL-1ß and TNF-α levels in BH group were significantly lower than those in the BR group (P < 0. 01). Conclusion: HRS can attenuate intestine injury induced by extensive burn after delayed fluid resuscitation. It can decrease the oxidative injury following extensive burn and delay fluid resuscitation, and it can also suppress the generation of proinflammatory cytokine IL-1ß and TNF-α.