Influence of adenovirus carrying Galectin-9 gene on differentiation and proliferation of CD4+CD25+ regulatory T cells in mice with heart transplantation / 第二军医大学学报

ABSTRACT

Objective: To explore the influence of adenovirus carrying Galectin-9 gene on the survival of cardiac transplant and the differentiation and proliferation of CD4+ CD25+ Treg cells in mice receiving heterotopic heart transplantation. Methods: Neck heterotopic heart transplantation models were established with male BALB/c→C57BL/6 mice by Cuff method, and they were randomly divided into 3 groups (n=10) control group (received 0.9% sodium chloride solution after operation), adenovirus group, and pAdeasy™ Galectin-9 treatment group. The survival time of the graft hearts was recorded. The spleens of mice were collected when the graft hearts stopped beating or 14 days after operation) the mononuclear cells were isolated and the proportion of CD4+ CD25+ Treg cells was determined by flow cytometry. Treg cell related cytokines (IL-10, TGF-β1) in the peripheral blood were assayed by ELISA. Results: The cardiac allografts in pAdeasy™ Galectin-9 group survived significantly longer than those in the other two groups (P<0.01), and the proportion of CD4+ CD25+ Treg cells in CD4+ T cells was significantly higher (P<0.01). The levels of IL-10 and TGF-β1 were increased in pAdeasy™ Galectin-9 group compared with control group and adenovirus group (P < 0.01). Conclusion: pAdeasy™ Galectin-9 can promote the proliferation and differentiation of CD4+ CD25+ Treg cells in vivo, and can greatly improve the survival time of cardiac allograft after heart transplantation in mice.