Efficacy of intraperitoneal sustained-release chemotherapy with 5-FU on mice bearing H22 ascitic tumor / 第二军医大学学报

ABSTRACT

Objective: To evaluate the inhibitory effect of intraperitoneal sustained-release chemotherapy with 5-FU on the growth of H22 ascitic tumor in mice. Methods: Mouse H22 ascitic tumor model was established by intraperitoneal injection of 0.2 ml H22 ascitic cells (4 × 106 cells) and the animals were subsequently divided into 4 groups randomly, namely, the saline control group (received saline), peritoneal chemotherapy group (received common 5-FU), sustained-release chemotherapy group (received sustained-release 5-FU), and negative control group (received control sustained-release agent). The survival times of the mice were recorded in all groups. The apoptosis rates of H22 ascitic cells were analyzed with flow cytometry 9 and 12 days after injection of H22 cells and the proliferation index was calculated. Electron microscope was used to observe H22 cells 12 days after peritoneal injection. Results: The average survival time of peritoneal chemotherapy group ([13.7±1.7] d) was significantly shorter than that of sustained-release chemotherapy group ([15.3±2.0]d) (P<0.05), but was longer than those of saline control group and negative control group (P<0.05), with no significant difference between the latter 2 groups. The apoptosis rate of H22 cells in sustained-release chemotherapy group was lower than that in peritoneal chemotherapy group (8.1±0.9 vs 16.5±1.7, P<0.05) 9 days after injection of H22 cells, but was higher than the latter 12 days after injection (10.1±1.3 vs 7.6±0.8, P<0.05); besides, they were both higher than those of saline control and negative control groups (P<0.05), with no significant difference found between the latter 2. Electron microscopic results showed that some H22 cells in peritoneal chemotherapy group and sustained-release chemotherapy group had a typical apoptosis appearance, and most cells in control group remained unchanged. Conclusion. Intraperitoneal sustained-release chemotherapy using 5-FU can prolong the survival time of mice bearing tumors and can inhibit the growth of H22 ascitic tumors for a longer period of time.