Expression of Fas ligand and T cell intracellular antigen-1 during acute after renal transplantation / 第二军医大学学报

ABSTRACT

Objective: To study the intrarenal expression of Fas ligand (FasL) and T cell intracellular antigen-1 (TIA-1) protein during acute rejection after renal transplantation. Methods: Immunohistochemical technique was used to detect FasL and TIA-1 expression in 32 transplanted renal samples,2 living donor kidneys and 8 adjacent samples of renal cancer (including 14 acute rejection samples, 15 chronic and 13 non-rejection ones according to Banff criteria). Results: The positive FasL staining was observed mainly in cytoplama of renal tubule epithelia, and the positive rate was significantly higher in acute rejection samples than in others(P<0.01). TIA-1 was found in both tubular epithelial cells and interstitial infiltrating lymphocytes, occasionaly in small arterial endothelial cells. The positive staining rate of TIA-1 acute rejection samples was also higher than those of other samples(P<0.05). The expression of FasL and TIA-1 in acute rejection samples was positively correlated with the severity of tissue damages. Conclusion: Over expression of FasL and TIA-1 in acute rejection renal allograft tissues may indicate the activation of T cells,and its detection may be helpful for early diagnosis of acute rejection.