Establishment of an orthotopic autologous liver transplantation model with bile ducts ischemia-reperfusion injury in rats / 第二军医大学学报

ABSTRACT

Objective: To establish an orthotopic autologous liver transplantation model with bile duct ischemia-reperfusion injury in rats, so as to make it easy for the operation. Methods: Eighty SD rats were used to establish the models. The method used for this model was the same as the one for orthotopic liver transplantation, except for the blood vessel anastomosis and the constant pressure perfusion of portal vein and abdominal aorta. The portal vein and the hepatic artery reperfusion were controlled by artery clamp. Results: The successful rate of the operation for establishing the model was 95%(76/80) and the anhepatic phase was (16±2) min. The periods of hot and cold ischemia and reperfusion could be accurately controlled and the effect of double constant pressure perfusion was good. There was no erythrocyte in the capillary of bile ductal wall under the light microscope after the reperfusion. Conclusion: This technique, simulating the whole process of liver transplantation, is simple and has high successful rate. Besides, it rules out the influence of immune agent on bile ducts impairment, and better reflects the pathophysiological process of bile ducts ischemia and reperfusion injury,providing a novel approach for investigation of bile ducts injury during liver transplantation.