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Influence of tanshinone II a on expressins of cosnic and aopp in kidney tissue of allergic purpura nephritis mice and its mechanism / 吉林大学学报(医学版)
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-841659
Biblioteca responsável: WPRO
ABSTRACT

Objective:

To explore the influence of tanshinone 11 A on the expressions of molecular chape rone Cosmc. advanced oxidation protein products (AOPP) in the kidney tissue of the allergic purpura nephritis mice and the levels of extracellular signal-regulated kinase/mitogen activated protein kinase (ERK/MAPK) signaling pathway proteins, and to clarify mechanism of tanshinone 11 A in the treatment of allergic purpura nephritis.

Methods:

A total of 38 mice were divided into control group∗ model group (allergic purpura nephritis model) and tanshinone 11 A group (allergic purpura nephritis model • tanshinone 11 A treatment), with 12 in each group; the other 2 mice were used to verify the success of modeling. The urine red blood cell counts and urine protein levels of the mice in various groups were measured, and the pathomorphology of kidney tissue of the mice in various groups were observed by periodate schiff-reaction (PAS) staining. The AOPP protein levels in the kidney tissue of the mice were determined by enzyme linked immunosorbent assay (ELISA), and the ERK and phosphorylated ERK (p-ERk) protein expression levels were determined by Western blotting method. The expression levels of ERK. p-ERK and Cosmc mRNA in kidney tissue of the mice were determined by RT-PCR.

Results:

In control group, there was no hyperplasia of glomerular basement membrane and no glomerular sclerosis; in model group, the proliferation of glomerular basement membrane was obvious∗ the inflammatory cells were infiltrated, and glomerular sclerosis appeared; in tanshinone 11 A group, glomerular basement membrane hyperplasia and inflammatory infiltration were not obvious in the mice, there was only small amount of infiltrated inflammatory cells. Compared with control group, the urine red blood cell count, the 24 h urine protein level, the p ERK mRNA and AOPP protein levels of the mice in model group and tanshinone 11 A group were increased ( P<0. 05 or P<0. 01); the Cosmc mRNA expression levels were decreased (P<0. 01). Compared with model group, the urine red blood cell count. 24 h urine protein level, the p-ERK and AOPP protein levels in the kidney tissue of the mice in tanshinone 11 A group were decreased (P<0. 05 or P<0. 01). and the Cosmc mRNA expression level was increased (PmRNA expression level in the kidney tissue was negatively correlated with the expression levels of AOPP protein and the expression level of p-ERK protein (r= 0. 573. P<0. 01; r= 0. 602. P<0. 01) . and the level of AOPP protein was positively correlated with the expression level of p-ERK protein ( r=0. 614. Pplay a role in the treatment of allergic purpura nephritis in the mice by lowering the levels of Cosmc. increasing the AOPP in kidney tissue and activating the ERK MAPK signaling pathways.

Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Jilin University(Medicine Edition) Ano de publicação: 2019 Tipo de documento: Artigo
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Jilin University(Medicine Edition) Ano de publicação: 2019 Tipo de documento: Artigo
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