Stem cell-like properties of glioma cells undergoing epithelial to mesenchymal transition and inhibitory effect of resveratrol on their stem cell-like properties / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the stem cell-like properties of glioma U87 cells undergoing epithelial to mesenchymal transition (EMT), and to clarify the inhibitory effect of resveratrol (Res) on their stem cell-like properties. Methods; The glioma U87 cells were treated with transforming growth factor-β1 (TGF-β1) to induce EMT, and the morphology of U87 cells was observed under inverted microscope. Western blotting method was used to detect the expression levels of mesenchymal markers (N-cadherin, Vimentin) and EMT-inducing transcription factors (Snail, Slug, Zebl, Twistl). After the EMT of glioma cells was confirmed, the experiment was divided into control group (U87 cells didn't receive any treatment), EMT group (the glioma cells presented EMT induced by TGF-β1) and Res treatment group (the glioma cells with EMT were treated with Res). The number of secondgeneration gliospheres and the expression levels of stem cell markers (Bmil and Sox2) in glioma U87 cells in various groups were detected. Results: The glioma U87 cells treated with TGF-β1 for 48 h showed dispersed, elongated, and similar to the appearance of fibroblasts. The expression levels of N-cadherin, Vimentin, Snail, Slug, Zebl and Twistl in glioma cells in different concentrations of TGF-β1 groups were significantly increased, suggesting the presence of EMT in glioma U87 cells. Compared with control group, the number of second-generation gliospheres in the U87 cells in EMT group was increased (P<0. 01), and the expression levels of stem cell markers (Bmil and Sox2) in the glioma U87 cells in EMT group were markedly increased (P<0. 05 or P<0. 01). Compared with EMT group, the number of second-generation gliospheres in the glioma U87 cells in Res treatment group was significantly decreased (P<0. 01), and the expression levels of Bmil and Sox2 in the glioma U87 cells in Res treatment group were decreased (P < 0. 01). Conclusion: The glioma U87 cells undergoing EMT exhibit the stem cell-like properties, and Res can inhibit their stem-like properties.