Effect of melatonin on radiosensitivity of non-small cell lung cancer H1299 cells / 吉林大学学报(医学版)

ABSTRACT

Objective: To detect the effects of melatonin (MLT) combined with radiotherapy on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) H1299 cells, and to explore the role of MLT in regulating the radiosensitivity of H1299 cells. Methods: The H1299 cells were divided into control group, MLT group, irradiation (IR) group and IR+MLT group. The H1299 cells in control group didn't receive any treatment, the H1299 cells in MLT group were treated with different concentrations of MLT (100 and 500 μmol · L-1), the H1299 cells in IR group were exposed to 6 Gy137 Cs γ-ray, and the H1299 cells in IR+MLT group were treated with different concentrations of MLT (100 and 500 μmol · L-1) and exposed to 6 Gy137 Cs γ-ray. Colony formation assay was used to detect the clone numbers of H1299 cells in various groups; Flow cytometry was applied to detect the apoptotic rates of H1299 cells at 24 and 48 h after irradiation; Western blotting method was performed to detect the phosphorylation levels of nuclear factor kappa B (NF-KB) protein in the H1299 cells in various groups. Results: The colony formation assay results showed that compared with IR group, the clone number of H1299 cells in IR+MLT group was significantly decreased (i=7. 234, P 0. 05). The Western blotting results showed that the phosphorylation level of NF-κB protein in the H1299 cells in IR+MLT group was lower than that in IR group. Conclusion: MLT combined with radiotherapy has obviously inhibitory effect on the human H1299 cells and can increase the radiosensitivity of H1299 cells.