Gene expression profiling of light-induced retinal degeneration in phototransduction gene knockout mice
Experimental & Molecular Medicine
; : 495-504, 2008.
Article
em En
| WPRIM
| ID: wpr-84653
Biblioteca responsável:
WPRO
ABSTRACT
Exposure to light can induce photoreceptor cell death and exacerbate retinal degeneration. In this study, mice with genetic knockout of several genes, including rhodopsin kinase (Rhok-/-), arrestin (Sag-/-), transducin (Gnat1-/-), c-Fos (c-Fos-/-) and arrestin/transducin (Sag-/-/Gnat1-/-), were examined. We measured the expression levels of thousands of genes in order to investigate their roles in phototransduction signaling in light-induced retinal degeneration using DNA microarray technology and then further explored the gene network using pathway analysis tools. Several cascades of gene components were induced or inhibited as a result of corresponding gene knockout under specific light conditions. Transducin deletion blocked the apoptotic signaling induced by exposure to low light conditions, and it did not require c-Fos/AP-1. Deletion of c-Fos blocked the apoptotic signaling induced by exposure to high intensity light. In the present study, we identified many gene transcripts that are essential for the initiation of light-induced rod degeneration and proposed several important networks that are involved in pro- and anti-apoptotic signaling. We also demonstrated the different cascades of gene components that participate in apoptotic signaling under specific light conditions.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Retina
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Degeneração Retiniana
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Transducina
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Genes fos
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Apoptose
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Camundongos Knockout
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Análise de Sequência com Séries de Oligonucleotídeos
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Perfilação da Expressão Gênica
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Subunidades alfa de Proteínas de Ligação ao GTP
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Receptor Quinase 1 Acoplada a Proteína G
Limite:
Animals
Idioma:
En
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2008
Tipo de documento:
Article