Bone morphogenetic protein-9 regulates expression of long non-coding RNAs in breast cancer cells / 肿瘤

ABSTRACT

Objective: To screen out the long non-coding RNAs (LncRNAs) related to bone morphogenetic protein-9 (BMP-9) expression, and to investigate the role of BMP-9 in the growth, differentiation, migration and apoptosis of breast cancer cells by regulating the expression of LncRNA. Methods: MDA-MB-231 cells were infected with the recombinant adenovirus Ad-BMP-9 carrying whole BMP -9 gene (named as MDA-MB-231/BMP-9 cells) or the empty vehicle adenovirus Ad-GFP carrying green fluorescent protein (GFP) gene (named as MDA-MB-231/GFP cells), respectively. Microarray technology was used to detect the difference in LncRNAs expression between MDAMB- 231/BMP-9 and MDA-MB-231/GFP cells. The changes of LncRNA LINC00443, LINC00638, LINC00486, RHNO1, SERHL, HOXA11-AS, IQCA1, LINC00461, LOC440173, LHFPL, ANKRD36BP2, BVES-AS1, LINC00937 and LINC00608 were validated by real-time fluorescent quantitative PCR. The biological funcation and related pathways of the above LncRNAs were analyzed by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Results: The expression level of BMP-9 mRNA in MDA-MB-231/BMP-9 cells was significantly higher than that in MDA-MB-231/GFP cells (as the control). Compared with the control group, the expression levels of LncRNAs in MDA-MB-231/BMP-9 cells changed significantly. The expression levels of LINC00443, LINC00638 and LINC00486 were up-regulated, while the expression levels of IQCA1, LINC00461, LOC440173, LHFPL and ANKRD36BP2 were down-regulated. The altered LncRNAs participated in the formation of cytoskeleton, cell membrane and so on, or played roles as signal molecules in intercellular signal transduction. Conclusion: The expression profile of LncRNAs in MDA-MB-231 cells with BMP-9 overexpression is significantly changed, indicating that LncRNAs may play key roles in the proliferation and invasion of breast cancer MDA-MB-231 cells regulated by BMP-9.