Establishment of an orthotopic transplant tumor model of murine lung cancer with stable expression of human IDO gene in mice / 肿瘤

ABSTRACT

Objective: To establish an orthotopic transplant tumor model of murine lung cancer with stable expression of human indoleamine-2, 3-dioxygenase (IDO) gene in mice. Methods: The murine lung cancer 2LL-EGFP-IDO cells with stable expression of IDO gene were transplanted into the lung or subcutaneous tissue of mice. The survival time of mice was observed. The tumorigenesis of murine lung cancer cells in lung was detected by pathological examination. The orthotopic transplant tumor model of lung cancer in mice was treated with 0.9% NaCI (as a model group), Chinese medicinal formula Feiji Recipe, IDO inhibitor l-methyl tryptophan (l-MT) or paclitaxel, respectively. The dynamic change of growth of lung cancer was observed by in vivo optical imaging. The expression level of IDO protein in the orthotopic transplant tumor of murine lung cancer was detected by Western blotting. Results: The survival time of mice-bearing orthotopic transplant tumor of murine lung cancer was shorterned as compared with that of the mice-bearing subcutaneous transplant tumor (P = 0.002). The pathologic result showed that tumorigenesis could be observed in the lung in mice. As compared with the model group, the growth of orthotopic transplant tumor of lung cancer was inhibited by Feiji Recipe, l-MT and paclitaxel (all P < 0.05). The expression level of IDO protein in the model group was higher than those in high-dose (100 mg·g-1d-1) Feiji Recipe, low-dose (50 mg·g-1d-1) Feiji Recipe and 1-MT groups (all P < 0.05). Conclusion: The orthotopic transplant tumor model of murine lung cancer 2LL-EGFP-IDO cells in mice can be successfully established, and the growth of transplant tumor and the IDO expression level can be inhibited by Feiji Recipe and l-MT.