Chemical constituents from Calystegia sepium / 中草药

ABSTRACT

Objective To study the chemical constituents from Calystegia sepium. Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20, ODS column chromatography, preparative HPLC and so on. Their structures were determined on the basis of physicochemical properties and their spectroscopic data, as well as literatures. Results A total of 20 compounds were separated and identified as tyrosol (1), quercetin-3-O-β-D-glucopyranoside (2), scutellarein-7-O-β- glucopyranoside (3), kaempferol-3-O-β-D-glucopyranoside (4), kaempferol-3-O-galactoside (5), kaempferol-3-O-β-robinobioside (6), nicotiflorin (7), rutin (8), uridine (9), tachioside (10), 2-methoxy-4-(2’-hydroxyethyl)-phenyl-1-O-β-D-glucopyranoside (11), 4-hydroxyphenethylol-4-O-β-D-glucopyranoside (12), polybotrin (13), pyridine-3,5-dicarboamide (14), skimmin (15), benzylalcohol-β-D-glucopyranoside (16), 5-hydroxyl-2-hydroxymethylpyridine (17), picein (18), 5-hydroxymethyl-furaldehyde (19), and sessiline (20). Conclusion Compounds 1-20 are isolated from Calystegia sepium for the first time.