Serum metabolomics reasearch of Tiansi Liquid on chronic stress rats utilizing NMR metabolomics / 中草药

ABSTRACT

Objective To explore the serum metabolomics mechanism of Tiansi Liquid on chronic stress rats. Methods The chronic unpredictable mild stress model with ovariectomization was utilized by giving Tiansi Liquid 3.5 g/kg, detection was carried out by Morris water maze and NMR metabolomics after six weeks of administration. Results The Morris water maze result showed that, compared with the control group, the distance to zone platform prolonged (P < 0.01) and the times of passing platform decreased (P < 0.05) in model group. While, the distance to zone platform shortened (P < 0.01) and the times of passing platform increased in treatment group compared with the model group. The NMR metabolomics showed that compared with the control group, the contents of alanine, allantoin, arginine, creatine, pyruvate, and serine were increased in model group, and the contents of asparagine, carnitine, glycerol, N,N-dimethylglycine, N-acetylglutamine, threonine, and valine were decreased in model group. The contents of glucose, glutamine, and methylhistidine in model group show different trends at different chemical shift. Compared with model group, the content of arginine and alanine were decreased in treatment group. Compared with the control group, the content of valine, arginine, glutamine, acetoacetate, asparagine, lysine, glycerol, carnitine, and threonine were decreased in treatment group, while the content of allantoin was increased in treatment group. Conclusion The main metabolomics effect of Tiansi Liquid on chronic stress rats was amino acid metabolism.