Quality evaluation research of Berberidis Radix formulations based on standard decoction () / 中草药

ABSTRACT

Objective To establish the quality evaluation system of Berberidis Radix (BR) standard decoction. Methods According to the preparation requirements of BR standard decoction, 15 batches of BR standard decoction powder was prepared, and, the index components in BR standard decoction was identified by using TLC chromatography. The content of index components was then detected by using HPLC, and the transfer rate of the index composition and the yield of the paste were calculated. Using infrared spectrometer to scan 15 batches of three standard decoctions. The infrared fingerprint spectrum was established, and the correlation between each batch was analyzed by using the two-index sequence analysis method. UV-vis spectrophotometer was used to respectively scan 15 batches of three needle standard medicinal broth for, establishing ultraviolet control map. The similarity between the fingerprint of sample and control was analyzed by SPSS software. Results The yield of 15 batches of standard decoction was 3.42%-6.36%. The content of berberine hydrochloride was 7.53%-13.98%, and the total transfer rate was 39.64%-73.62%. The same colored fluorescent spots were appeared at the corresponding positions of BR standard decoction, the berberine hydrochloride control, and BR control solution and the spots were in the same position of the same silica gel G thin layer. The lower limit of total peak rate of IR fingerprint was 50.0%, and the variation peak rate was 0.0%-62.5%. The similarity of UV fingerprints was all greater than 0.9, and the variation acceptable range was 0.768-1.000. Conclusion A stable and reliable method for the comprehensive quality evaluation of BR standard decoction was established, which can provide reference for the quality control of BR formula granules.