Effect of ginsenoside Rh2 on drug resistance sensitivity of human gastric cancer SGC7901/ADR cells / 中草药

ABSTRACT

Objective To observe the effect of ginsenoside Rh2 (G-Rh2) on the proliferation, cell cycle and chemotherapy sensitivity of human gastric cancer cell line SGC7901/ADR. Methods MTT assay was used to detected the effects of G-Rh2 and adriamycin (ADR) on the proliferation of drug-resistant SGC7901/ADR cells and to calculate the reversal fold (RF). Flow cytometry was selected to detect The effects of G-Rh2 on cell cycle. the effects of G-Rh2 on the expression of P-gp and Bcl-2 proteins in drug-resistant SGC7901/ADR cells was detected by Western blotting. Results Compared with the IC50 value (54.52 μmol/L) after the ADR single drug treatment, the IC50 value (30.14 μmol/L) of the cells treated with G-Rh2 and ADR decreased significantly, and RF was 1.81. G-Rh2 combined with ADR arrested the cell cycle in G2/M phase and significantly decreased the protein expression of P-gp and Bcl-2(P < 0.05). Conclusion G-Rh2 combined with ADR could increase the chemosensitivity of SGC7901/ADR cells, which may inhibit the proliferation of gastric cancer cells by blocking cell cycle and increasing apoptosis.