Comparative study of different extract methods for protein and peptides from Chinese medicine bovine bone marrow / 中草药

ABSTRACT

Objective: To study the extraction method, structure information, and antimicrobial activity of the protein and polypeptide components from Chinese medicine bovine bone marrow (BBM) for improvement of its medicinal value. Methods: Ten kinds of extraction and three kinds of preparation methods were used to take the protein content, extraction rate, degree of hydrolysis, antimicrobial activity, and molecular weight as investigation index to screen optimal extraction ability and preparation parts of bovine bone marrow protein (BBMP) and polypeptide. Results: The comprehensive evaluation index of protein extracted in water, NaCl, and Tris-HCl solutions was high, their contents were (52.20 ± 0.58), (50.19 ± 0.21), and (43.21 ± 0.05) mg/mL, respectively. The antimicrobial ability was related to protein content. The SDS-PAGE analysis showed that the proteins from BBM contained approximately secondary subunits which related to protein and peptide with molecular weight in the range of 66 000 and 4 100- 10 000. The LC/MS data showed that the molecular weight of peptide extracted in water, 0.5 mol/L sodium chloride, and 30% (NH4)2SO4 solutions were 1 027.84-9 916.32, 1 131.17-4 989.95, and 1 150.46-9 917.00, respectively, and the results were basically consistent with 1-DE electrophoresis bands. Conclusion: Water extraction method is simple, and fast with relatively low cost, which is suitable for industrial production of BBMP. The SDS-PAGE and LC/MS method is adopted to analyze and provide a theoretical basis for construction of BBMP fingerprint.