Effect of chlorogenic acid at high concentration on lipid accumulation and oxidative stress injury in L02 cells / 中草药

ABSTRACT

Objective To investigate the effects of chlorogenic acid (CGA) at high concentration ( > 100 μmol/L) on the lipid accumulation and oxidative stress in L02 cells under normal and non alcoholic liver disease (NAFLD) status induced by oleic acid (OA) and palmic acid (PA) treatment. Methods L02 cells were treatment by OA (666 μmol/L)-PA (333 μmol/L) for 24 h to induce intracellular steatosis. After normal and OA-PA treated cells were treated by CGA (0, 0.5, 1, and 2 mmol/L) for 24 h, the intracellular contents of lipid droplets and reactive oxygen species (ROS) were determined by Oid red staining and fluorospectrophotometry, respectively. And the mRNA and protein expression levels of SREBP-1C, PNPLA3, and CYP2E1 were detected by Real-time PCR and Western blotting, respectively. Results CGA treatment dose-dependently increased the levels of intracellular lipid droplets and ROS, as well as the mRNA and protein expression levels of SREBP-1C, PNPLA3, and CYP2E1 in normal and OA-PA treated L02 cells. Conclusion CGA treatment at high concentration can accelerate the lipid accumulation and oxidative stress injury in L02 cells under normal and NAFLD status.