Based on sequencing of 16 S rRNA gene elaborate effect of Coptis chinensis on intestinal microbial diversity in type 2 diabetes mellitus / 中草药

ABSTRACT

Objective To explore the effect of Coptis chinensis on the intestinal microflora diversity of rats with type 2 diabetes mellitus and further elucidate the mechanism of C. chinensis in treatment of type 2 diabetes mellitus. Methods SD rats were randomly divided into control group (CON), model group (M), C. chinensis group (HL), and positive control group (POS), 10 in each group respectively. Type 2 diabetes mellitus model was caused by special diet fed and ip injection of STZ together. CON with M, HL, and POS was respectively given gavage volume 0.5% CMC-Na, 4.36 g/kg C. chinensis water extract and 0.097 g/kg of metformin. The body weight and blood glucose were monitored once a week separately. After four weeks of administration, the oral glucose tolerance and insulin level were measured. After four weeks of administration, the contents of rectum were collected and sequenced by a 16 S rRNA gene sequencing. Results A total of 951OTU, 15 Phylum, 25 Class, 43 Order, 69 Family, 182 Genus and 357 Species were obtained. At the level of Phylum, the content of Actinobacteria in M was significantly higher than that in CON and HL (P < 0.05), while Deferribacteres was only detected in M. In M, Firmicutes, Spirochaetae, Tenericutes, and Elusimicrobia were higher than CON. Interestingly, they were all reduced after giving C. chinensis, meanwhile the abundance of Proteobacteria, Fusobacteria and Verrucomicrobia in HL were slightly higher than that in CON and M. Conclusion Actinobacteria and Deferribacteres were probably potential markers in type 2 diabetes mellitus. After administrating C. chinensis, the change of intestinal microflora diversity revealed C. chinensis could improve the intestinal microflora disorder of type 2 diabetes mellitus.