Purification of total flavonoids extracted from Astragali Complanati Semen using D-101 macroporous adsorption resin / 中草药
Chinese Traditional and Herbal Drugs
; (24): 3342-3346, 2017.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-852589
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To optimize the purification technology of total flavonoids from Astragali Companati Semen (ACS) by macroporous adsorption resin.Methods:
The process parameters of water decoction extraction of ACS and purification of total flavonoids extracted from ACS by D-101 macroporous adsorption resin were optimized by orthogonal experiment design method.Results:
The optimal extraction process were as follows water decoction extract for three times, each time 0.5 h, water addition of 10, 6, and 6 times amount of the medicine respectively. The optimized technology conditions of D-101 macroporous adsorption resin were as follows The flavonoids concentration of sample liquid of ACS was about 1.0 mg/mL, the diameter ratio was 1∶5, the sample flow rate was 1 BV/h, the rate of sample weight was 0.6 g/mL (herbs/resin); The velocity of water elution was 1 BV/h and its elution volume was 4 BV; The elution concentration, elution velocity, and elution volume were 70%, 1 BV/h, and 5 BV, respectively. The content paste rate of made ACS of total flavonoids was 2.65%, the mass fraction of total flavonoids was 56.24%, and the process transfer rate was 68.37%.Conclusion:
The method is simple and feasible for purification of total flavonoids extracted from ACS.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2017
Tipo de documento:
Artigo